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1 Reactivity: Does the BG test kit react to analytes other than (1→3)-β-D-Glucan ?
No. Only (1→3)-β-D-Glucan has been shown to be reactive to . Factor G, the beta-glucan sensing component is very specific for the (1→3)-β-linked glucan structure.
BG assay works as an amplification cascade that allows a small stimulus to produce a large output signal. (1→3)-ß-D-glucan activates a protease zymogen that, in turn, activates many molecules of another protease zymogen. This protease cleaves a chromogenic substrate, producing the increase in the optical density at 405 nanometers. This amplification accounts for the extreme sensitivity of our kit.
Our kit could detect as little as 10 picograms of (1→3)-β-D-glucan per mL.
Our kit has two different categories: one is BG-110T, it runs in 96-well microplates, is similar to standard microplate-based EIAs but it is a homogeneous, in-well assay, there are no washing steps. The other one is BG-50T run in depyrogenated/pyrogen-free flat tube.
5 Assay: Which instrument is suitable for the BG assay?
For BG-110T, an incubating microplate reader is required. For BG-50T, a Goldstream Kinetic Reader is required, all the BG assay is run at 37°C.
6 Assay: What software is required to run the BG assay?
For BG-110T, any microplate reader software that can calculate Vmean over the 40 minute incubation period should work. For BG-50T, the software of the Microbiology Detection System is specially designed for use with our Goldstream Kinetic Reader.
7 Assay: How long does it take to react?
For BG-110T and BG-50T, reaction can take 30-40 minutes, initially.
8 Assay: How are the results calculated?
For BG-110T, the microplate reader software automatically calculates the mean rate of optical density change (milli-absorbance units per minute) for all points between 0 and 40 minutes. For BG-50T, the Microbiology Detection System automatically calculates the mean rate of optical density change (milli-absorbance units per 10s) for all points between 0 and 40 minutes.
9 Assay: What standard is used?
Pachyman standard, a linear (1,3)-beta-glucan, is provided in each kit.
10 Interpretation: Does a positive value always mean that the patient has a fungal infection?
No. The entire clinical context must be considered. A number of potential sources of (1→ 3)-ß -D-glucan contamination should be considered. These include recent surgery with invasive exposure to cotton surgical sponges, etc., and/or contaminated blood fractionation products. The BG assay is indicated as an adjunct to diagnosis and is to be considered, with other clinical signs and symptoms, in the development of a diagnosis.
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